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RBM17 Recombinant antibody

RBM17 Uni-rAbTM Recombinant Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

230125F6

Cat no : 82889-1-RR

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Recombinant antibody (82889-1-RR)

    IP experiment of MCF-7 using 82889-1-RR

    IP result of anti-RBM17 (IP:82889-1-RR, 4ug; Detection:82889-1-RR 1:10000) with MCF-7 cells lysate 1470 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM17 Recombinant antibody (82889-1-RR)

    IF Staining of HepG2 using 82889-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM17 antibody (82889-1-RR, Clone: 230125F6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • Flow cytometry (FC) experiment of HepG2 cells using RBM17 Recombinant antibody (82889-1-RR)

    FC experiment of HepG2 using 82889-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBM17 Recombinant antibody (82889-1-RR, Clone:230125F6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82889-1-RR

    Affinity and Kinetic Characterization of 82889-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-1-RR against Human RBM17 were performed. The affinity constant is 1.54 nM.

Tested Applications

Positive IP detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82889-1-RR targets RBM17 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985
Full Name RNA binding motif protein 17
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Protocols

Product Specific Protocols
IF protocol for RBM17 antibody 82889-1-RRDownload protocol
IP protocol for RBM17 antibody 82889-1-RRDownload protocol
FC protocol for RBM17 antibody 82889-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols