CoraLite® Plus 488-conjugated RBM15B Monoclonal antibody

RBM15B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C2C11

Cat no : CL488-67506

Synonyms

RBM15B, RNA-binding motif protein 15B, 1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3, OTT3, Putative RNA-binding protein 15B

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Validation Data Gallery

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  FC experiment of HepG2 using CL488-67506

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RBM15B (CL488-67506, Clone:1C2C11) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67506 targets RBM15B in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM15B fusion protein Ag17853
• Full Name: RNA binding motif protein 15B
• Calculated Molecular Weight: 890 aa, 97 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC001367
• Gene Symbol: RBM15B
• Gene ID (NCBI): 29890
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011