Validation Data Gallery
|Positive WB detected in
|HeLa cells, A549 cells, K-562 cells, COLO 320 cells, LNCaP cells, HEK-293 cells, Jurkat cells, THP-1 cells
|Positive IHC detected in
|human colon cancer tissue, human colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
67506-1-Ig targets RBM15B in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.
|Host / Isotype
|Mouse / IgG1
|RBM15B fusion protein Ag17853
|RNA binding motif protein 15B
|Calculated molecular weight
|890 aa, 97 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).
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