CoraLite® Plus 488-conjugated RbAp46 Recombinant antibody

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  IF Staining of A431 using CL488-84330

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBBP7 antibody (CL488-84330, Clone: 241189D6 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-84330

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug Coralite® Plus 488 Rbbp7 Recombinant Antibody (CL488-84330, Clone:241189D6)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RbAp46 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-84330

Clone No.241189D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7

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Tested Applications

• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84330 targets RbAp46 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: retinoblastoma binding protein 7
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: NM_002893
• Gene Symbol: RBBP7
• Gene ID (NCBI): 5931
• RRID: AB_3673354
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16576
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.