RAD1 Monoclonal antibody

RAD1 Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E2

Cat no : 68637-1-Ig

Synonyms

HRAD1, RAD1, RAD1 homolog (S. pombe), REC1

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Validation Data Gallery

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  WB analysis using 68637-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68637-1-Ig

  IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: MKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68637-1-Ig targets RAD1 in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAD1 fusion protein Ag33257
• Full Name: RAD1 homolog (S. pombe)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC006837
• Gene Symbol: RAD1
• Gene ID (NCBI): 5810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.