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RAD1 Monoclonal antibody, PBS Only

RAD1 Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11E2

Cat no : 68637-1-PBS

Synonyms

HRAD1, RAD1, RAD1 homolog (S. pombe), REC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD1 Monoclonal antibody (68637-1-Ig)

    WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RAD1 Monoclonal antibody (68637-1-Ig)

    IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68637-1-PBS targets RAD1 in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD1 fusion protein Ag33257
Full Name RAD1 homolog (S. pombe)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC006837
Gene Symbol RAD1
Gene ID (NCBI) 5810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.