Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241018D12

Cat no : 80130-2-PBS

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

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Validation Data Gallery

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  WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

  Non-treated and PDGF treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (RPS6-phospho-S235/236 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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Product Information

80130-2-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001010
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)