Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody

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  WB analysis using 80130-2-RR

  Non-treated and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  WB analysis of NIH/3T3 using 80130-2-RR

  Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  WB analysis using 80130-2-RR

  Non-treated and PDGF (HZ-1215) treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236)) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 80130-2-RR

  Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) antibody (80130-2-RR, Clone: 241018D12) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

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  FC experiment of HeLa using 80130-2-RR

  1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody (80130-2-RR, Clone: 241018D12, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA .

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 80130-2-RR

Clone No.241018D12

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236

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Tested Applications

• Positive WB detected in: PDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells
• Positive IF/ICC detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells
• Positive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001010
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_3670467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P62753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling; Authors - Kunxiang Gong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (02-10-2025)

pS6 worked perfectly in my FFPE human brain cortical sections. Marking my BCs and Dns in TSC tissue.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Immunohistochemistry validation (1:100 dilution) in Human Brain Cortex (Cat no:80130-2-RR)