Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4E16

Cat no : 82534-1-RR

Synonyms

p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3

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Validation Data Gallery

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  WB analysis using 82534-1-RR

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82534-1-RR

  Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control.

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  FC experiment of HEK-293 using 82534-1-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody (82534-1-RR, Clone:4E16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells, NIH/3T3 cells, Calyculin A treated NIH/3T3 cells
• Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells

This antibody is equivalent to Thr980 in mice.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:11200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82534-1-RR targets Phospho-PERK/EIF2AK3 (Thr982) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• RRID: AB_3085359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publications

Species	Application	Title
human	WB	Vet Microbiol; Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replication; Authors - Yu Han