Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody, PBS Only

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  WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

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  WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

  Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody (82534-1-RR, Clone:4E16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

Phospho-PERK/EIF2AK3 (Thr982) {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82534-1-PBS

Clone No.4E16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3

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This antibody is equivalent to Thr980 in mice.

Product Information

82534-1-PBS targets Phospho-PERK/EIF2AK3 (Thr982) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZJ5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)