Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody, PBS Only

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E16

Cat no : 82534-1-PBS

Synonyms

EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse

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Validation Data Gallery

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  WB analysis of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

  Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

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Product Information

82534-1-PBS targets Phospho-PERK/EIF2AK3 (Thr982) in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)