CoraLite® Plus 488-conjugated Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-82534
Clone No.4E16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3, Phospho PERK/EIF2AK3 (Thr982), Phospho-PERK/EIF2AK3, Phospho-PERK/EIF2AK3 Thr982

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC (Intra) detected inCalyculin A treated HEK-293 cells

This antibody is equivalent to Thr980 in mice.

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82534 targets Phospho-PERK/EIF2AK3 (Thr982) in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

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