CoraLite® Plus 488-conjugated PURA Monoclonal antibody

PURA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6G4

Cat no : CL488-67305

Synonyms

Transcriptional activator protein Pur-alpha, Purine-rich single-stranded DNA-binding protein alpha, PURALPHA, PUR1, PUR ALPHA

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67305

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PURA (CL488-67305, Clone:1C6G4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67305 targets PURA in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PURA fusion protein Ag12164
• Full Name: purine-rich element binding protein A
• Calculated Molecular Weight: 322 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC036087
• Gene Symbol: PURA
• Gene ID (NCBI): 5813
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.