PURA Monoclonal antibody

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• KD/KO Validated

PURA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6G4

Cat no : 67305-1-Ig

Synonyms

PUR ALPHA, PUR1, PURA, PURALPHA

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Validation Data Gallery

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  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67305-1-Ig

  WB result of PURA antibody (67305-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, pig brain tissue, rat brain tissue, mouse brain tissue, L02 cells, LNCaP cells, human placenta tissue, rat cerebellum tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67305-1-Ig targets PURA in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PURA fusion protein Ag12164
• Full Name: purine-rich element binding protein A
• Calculated Molecular Weight: 322 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC036087
• Gene Symbol: PURA
• Gene ID (NCBI): 5813
• RRID: AB_2882567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.