Filter: AllWBIHCFC (Intra)

WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.
WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.
FC experiment of A549 using 67658-1-Ig (same clone as 67658-1-PBS)
1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.
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1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

EPCR/CD201 Monoclonal antibody, PBS Only

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 67658-1-PBS

Clone No.1F5C4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67658-1-PBS targets EPCR/CD201 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	EPCR/CD201 fusion protein Ag25628 Predict reactive species
Full Name	protein C receptor, endothelial (EPCR)
Calculated Molecular Weight	238 aa, 27 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC014451
Gene Symbol	PROCR
Gene ID (NCBI)	10544
RRID	AB_2882855
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UNN8
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.