EPCR/CD201 Monoclonal antibody

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  WB analysis using 67658-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67658-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67658-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67658-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A549 using 67658-1-Ig

  1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 67658-1-Ig

Clone No.1F5C4

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

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Tested Applications

• Positive WB detected in: human placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67658-1-Ig targets EPCR/CD201 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPCR/CD201 fusion protein Ag25628
• Full Name: protein C receptor, endothelial (EPCR)
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014451
• Gene Symbol: PROCR
• Gene ID (NCBI): 10544
• RRID: AB_2882855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UNN8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Signal Transduct Target Ther; Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.; Authors - Panpan Zhang