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EPCR/CD201 Monoclonal antibody

EPCR/CD201 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C4

Cat no : 67658-1-Ig

Synonyms

CD201, EPCR, PROCR, CCD41, CCCA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROCR Monoclonal antibody (67658-1-Ig)

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PROCR Monoclonal antibody (67658-1-Ig)

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PROCR Monoclonal antibody (67658-1-Ig)

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PROCR Monoclonal antibody (67658-1-Ig)

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67658-1-Ig targets EPCR/CD201 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPCR/CD201 fusion protein Ag25628
Full Name protein C receptor, endothelial (EPCR)
Calculated Molecular Weight 238 aa, 27 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC014451
Gene Symbol PROCR
Gene ID (NCBI) 10544
RRIDAB_2882855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
IHC protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Signal Transduct Target Ther

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Authors - Panpan Zhang
View Article