CoraLite®594-conjugated PPP4R1 Monoclonal antibody

PPP4R1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D12G12

Cat no : CL594-67392

Synonyms

MEG1, PP4(Rmeg), PP4R1, PPP4R1

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Validation Data Gallery

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  IF Staining of mouse liver using CL594-67392

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 PPP4R1 antibody (CL594-67392, Clone: 3D12G12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67392

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PPP4R1 (CL594-67392, Clone:3D12G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse liver tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67392 targets PPP4R1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP4R1 fusion protein Ag26750
• Full Name: protein phosphatase 4, regulatory subunit 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC060829
• Gene Symbol: PPP4R1
• Gene ID (NCBI): 9989
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.