Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 67392-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67392-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human liver cancer using 67392-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse liver using 67392-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of HeLa using 67392-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected in
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human liver cancer tissue, mouse liver tissue
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:6000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 67392-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67392-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of human liver cancer using 67392-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse liver using 67392-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HeLa using 67392-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PPP4R1 Monoclonal antibody
Catalog Number
67392-1-Ig
Citations
1
Dilutions
WB : 1:2000-1:6000 IHC : 1:250-1:1000 IF-P : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
