CoraLite® Plus 488-conjugated PPP1CB-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP1CB-Specific Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55136

Synonyms

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

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Validation Data Gallery

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  IF Staining of U2OS using CL488-55136

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PPP1CB-Specific antibody (CL488-55136) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of U2OS using CL488-55136

  1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 PPP1CB-Specific Polyclonal Antibody (CL488-55136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55136 targets PPP1CB-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein phosphatase 1, catalytic subunit, beta isoform
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: NM_002709
• Gene Symbol: PPP1CB
• Gene ID (NCBI): 5500
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.