PPP1CB-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP1CB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 55136-1-AP

Synonyms

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

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Validation Data Gallery

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  WB analysis using 55136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 55136-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 55136-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

55136-1-AP targets PPP1CB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein phosphatase 1, catalytic subunit, beta isoform
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: NM_002709
• Gene Symbol: PPP1CB
• Gene ID (NCBI): 5500
• RRID: AB_10837232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

Publications

Species	Application	Title
human	WB	PLoS Pathog; Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.; Authors - Zhanying Hu ; KD Validated
human	WB	Exp Eye Res; PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.; Authors - Yang Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-23-2022)

I have used this antibody for western blot in mice tissue sample (colon tissue). it is working great.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Colon tissue sample and caco2 cells