CoraLite® Plus 488-conjugated POTEA-Specific Recombinant antibody

POTEA-Specific Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241187F7

Cat no : CL488-84298

Synonyms

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

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Validation Data Gallery

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  FC experiment of HeLa using CL488-84298

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 POTEA-Specific Recombinant Antibody (CL488-84298, Clone:241187F7)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84298 targets POTEA-Specific in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: POTE ankyrin domain family, member A
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: NM_001002920
• Gene Symbol: POTEA
• Gene ID (NCBI): 340441
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.