PGK2 Monoclonal antibody, PBS Only

PGK2 Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D4F2

Cat no : 68228-1-PBS

Synonyms

PGKPS, PGKB, EC:2.7.2.3, 3D4F2

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Validation Data Gallery

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  WB analysis using 68228-1-Ig (same clone as 68228-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68228-1-Ig (same clone as 68228-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 68228-1-Ig (same clone as 68228-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 68228-1-Ig (same clone as 68228-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (68228-1-Ig, Clone: 3D4F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 68228-1-Ig (same clone as 68228-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human PGK2 (68228-1-Ig, Clone:3D4F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

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Product Information

68228-1-PBS targets PGK2 in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

• Tested Reactivity: Human, Rat, Pig, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGK2 fusion protein Ag20631
• Full Name: phosphoglycerate kinase 2
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC038843
• Gene Symbol: PGK2
• Gene ID (NCBI): 5232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.