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PGK2 Monoclonal antibody

PGK2 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3D4F2

Cat no : 68228-1-Ig

Synonyms

PGKPS, PGKB, EC:2.7.2.3, 3D4F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis using 68228-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis using 68228-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis of HuH-7 using 68228-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IF Staining of mouse testis using 68228-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (68228-1-Ig, Clone: 3D4F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using PGK2 Monoclonal antibody (68228-1-Ig)

    FC experiment of A549 using 68228-1-Ig

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human PGK2 (68228-1-Ig, Clone:3D4F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, human testis tissue, HuH-7 cells, Raji cells, MOLT-4 cells, pig heart tissue, rat heart tissue
Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68228-1-Ig targets PGK2 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

Tested Reactivity Human, Rat, Pig, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGK2 fusion protein Ag20631
Full Name phosphoglycerate kinase 2
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC038843
Gene Symbol PGK2
Gene ID (NCBI) 5232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Protocols

Product Specific Protocols
WB protocol for PGK2 antibody 68228-1-IgDownload protocol
IF protocol for PGK2 antibody 68228-1-IgDownload protocol
FC protocol for PGK2 antibody 68228-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols