PER1 Recombinant antibody

PER1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242096E3

Cat no : 84730-5-RR

Synonyms

period homolog 1 (Drosophila), PER, KIAA0482, hPER1, hPER

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Validation Data Gallery

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  IF Staining of NIH/3T3 using 84730-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84730-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84730-5-RR targets PER1 in IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PER1 fusion protein Ag4249
• Full Name: period homolog 1 (Drosophila)
• Calculated Molecular Weight: 1290 aa, 136 kDa
• GenBank Accession Number: BC028207
• Gene Symbol: PER1
• Gene ID (NCBI): 5187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].