CoraLite® Plus 647-conjugated PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13371

Synonyms

PARP1, Poly [ADP-ribose] polymerase 1, Poly [ADP-ribose] polymerase 1, processed C-terminus, Poly [ADP-ribose] polymerase 1, processed N-terminus, Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1, ARTD1, DNA ADP-ribosyltransferase PARP1, EC:2.4.2.-, EC:2.4.2.30, NAD(+) ADP-ribosyltransferase 1

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Validation Data Gallery

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  FC experiment of K-562 using CL647-13371

  1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated PARP1 Polyclonal antibody (CL647-13371)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using CL647-13371

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PARP1 (CL647-13371) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: K-562 cells, HeLa cells
• Positive FC detected in: K-562 cells, HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13371 targets PARP1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARP1 fusion protein Ag4193
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated Molecular Weight: 1014 aa, 113 kDa
• Observed Molecular Weight: 113-116 kDa, 89 kDa
• GenBank Accession Number: BC037545
• Gene Symbol: PARP1
• Gene ID (NCBI): 142
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.