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Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-30000 targets Rabbit IgG control in FC (Intra) applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N/A Predict reactive species |
| Full Name | Rabbit IgG control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883649 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | N/A |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. This antibody can be used as Rabbit IgG control in experiments.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Rabbit IgG control antibody CL647-30000 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
