CoraLite® Plus 488-conjugated P21 Polyclonal antibody

• Featured Product
• KD/KO Validated

P21 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

Cat no : CL488-10355

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-10355

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 P21 antibody (CL488-10355) at dilution of 1:100.

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  FC experiment of MCF-7 using CL488-10355

  1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human P21 (CL488-10355) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10355 targets P21 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P21 fusion protein Ag0368
• Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000275
• Gene Symbol: p21
• Gene ID (NCBI): 1026
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	FC (Intra)	Cardiovasc Res; Senescent CD8+ T cells: a novel risk factor in atrial fibrillation; Authors - Xiang Li