CoraLite® Plus 488-conjugated Neuroserpin Monoclonal antibody

Neuroserpin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4C6

Cat no : CL488-66997

Synonyms

Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12

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Validation Data Gallery

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  IF Staining of U-251 using CL488-66997

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 Neuroserpin antibody (CL488-66997, Clone: 1G4C6 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66997 targets Neuroserpin in IF/ICC applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neuroserpin fusion protein Ag28636
• Full Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018043
• Gene Symbol: Neuroserpin
• Gene ID (NCBI): 5274
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.