Neuroserpin Monoclonal antibody

Neuroserpin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1G4C6

Cat no : 66997-1-Ig

Synonyms

DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1

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Validation Data Gallery

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  WB analysis of pig brain using 66997-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66997-1-Ig (SERPINI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 66997-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 66997-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 66997-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Neuroserpin antibody (66997-1-Ig, Clone: 1G4C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue
• Positive IHC detected in: rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66997-1-Ig targets Neuroserpin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neuroserpin fusion protein Ag28636
• Full Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018043
• Gene Symbol: Neuroserpin
• Gene ID (NCBI): 5274
• RRID: AB_2882314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.

Publications

Species	Application	Title
mouse	WB,IF	Brain Pathol; Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.; Authors - Nicole R Higgins