NR1H4 Recombinant antibody

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  WB analysis using 81820-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81820-2-RR (NR1H4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 81820-2-RR

  WB result of NR1H4 antibody (81820-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR1H4 transfected HepG2 cells.

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  FC experiment of HepG2 using 81820-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NR1H4 Recombinant antibody (81820-2-RR, Clone:4B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 81820-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81820-2-RR against Human NR1H4 were performed. The affinity constant is 0.807 nM.

• Featured Product
• KD/KO Validated

NR1H4 Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 81820-2-RR

Clone No.4B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR

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Tested Applications

• Positive WB detected in: MKN-45 cells, HepG2 cells, HEK-293 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81820-2-RR targets NR1H4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NR1H4 fusion protein Ag21948
• Full Name: nuclear receptor subfamily 1, group H, member 4
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC130573
• Gene Symbol: NR1H4
• Gene ID (NCBI): 9971
• RRID: AB_3670508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96RI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)