NME2 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85074-2-RR (same clone as 85074-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 85074-2-RR (same clone as 85074-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01804-1

  Cytometric bead array standard curve of MP01804-1, NME2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85074-3-PBS. Detection antibody: 85074-2-PBS. Standard: Ag13913. Range: 1.563-200 ng/mL.

NME2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 85074-2-PBS

Clone No.242698C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF

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Product Information

85074-2-PBS targets NME2 as part of a matched antibody pair:

MP01804-1: 85074-3-PBS capture and 85074-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NME2 fusion protein Ag13913
• Full Name: non-metastatic cells 2, protein (NM23B) expressed in
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC002476
• Gene Symbol: NME2
• Gene ID (NCBI): 4831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22392
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.