NME2 Recombinant antibody

• 

  WB analysis using 85074-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293T using 85074-2-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NME2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 85074-2-RR

Clone No.242698C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85074-2-RR targets NME2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NME2 fusion protein Ag13913
• Full Name: non-metastatic cells 2, protein (NM23B) expressed in
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC002476
• Gene Symbol: NME2
• Gene ID (NCBI): 4831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22392
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.