NF-κB p65 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

NF-κB p65 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4C7

Cat no : 80979-1-PBS

Synonyms

NF κB, p65, p65; RELA, RELA, NFKB p65

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Validation Data Gallery

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  WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

  WB result of NF-κB p65 antibody (80979-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80979-1-RR (same clone as 80979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80979-1-RR (NF-κB p65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

  WB result of NF-κB p65 antibody (80979-1-RR; 1:13000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80979-1-RR (same clone as 80979-1-PBS)

  IP result of anti-NF-κB p65 (IP:80979-1-RR, 4ug; Detection:80979-1-RR 1:4000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-1080 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NF-κB p65 (80979-1-RR, Clone:4C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80979-1-RR against Human NF-κB p65 were performed. The affinity constant is 3.57 nM.

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Published Applications

• WB: See 1 publications below

Product Information

80979-1-PBS targets NF-κB p65 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NF-κB p65 fusion protein Ag1199
• Full Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011603
• Gene Symbol: NF-κB p65
• Gene ID (NCBI): 5970
• RRID: AB_2918923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Publications

Species	Application	Title
human,mouse	WB	Int Immunopharmacol; The ellagitannin metabolite urolithin C attenuated cognitive impairment by inhibiting neuroinflammation via downregulation of MAPK/NF-kB signaling pathways in aging mice; Authors - Peng Chen