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NF-κB p65 Recombinant antibody

NF-κB p65 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C7

Cat no : 80979-1-RR

Synonyms

NF κB, NFKB3, NF-κB p65, p65, RELA, Transcription factor p65



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, MOLT-4 cells, Jurkat cells, Raji cells, NIH/3T3 cells, HSC-T6 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

80979-1-RR targets NF-κB p65 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Protocols

Product Specific Protocols
WB protocol for NF-κB p65 antibody 80979-1-RRDownload protocol
IF protocol for NF-κB p65 antibody 80979-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols