NEIL1 Recombinant antibody, PBS Only

NEIL1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240322G10

Cat no : 83376-7-PBS

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

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Validation Data Gallery

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  WB analysis using 83376-7-RR (same clone as 83376-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83376-7-RR (NEIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83376-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 83376-7-RR (same clone as 83376-7-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NEIL1 Recombinant antibody (83376-7-RR, Clone:240322G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83376-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83376-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83376-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is 0.521 nM.

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Product Information

The immunogen of 83376-7-PBS is NEIL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEIL1 fusion protein Ag2793
• Full Name: nei endonuclease VIII-like 1 (E. coli)
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC010876
• Gene Symbol: NEIL1
• Gene ID (NCBI): 79661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)