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NEIL1 Recombinant antibody

NEIL1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240322G10

Cat no : 83376-7-RR

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEIL1 Recombinant antibody (83376-7-RR)

    WB analysis using 83376-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83376-7-RR (NEIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using NEIL1 Recombinant antibody (83376-7-RR)

    FC experiment of HEK-293 using 83376-7-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NEIL1 Recombinant antibody (83376-7-RR, Clone:240322G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83376-7-RR

    Affinity and Kinetic Characterization of 83376-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is 0.521 nM.

  • Affinity and Kinetic Characterization of 83376-7-RR

    Affinity and Kinetic Characterization of 83376-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, DU145 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83376-7-RR is NEIL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEIL1 fusion protein Ag2793
Full Name nei endonuclease VIII-like 1 (E. coli)
Calculated Molecular Weight 390 aa, 44 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC010876
Gene Symbol NEIL1
Gene ID (NCBI) 79661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)

Protocols

Product Specific Protocols
WB protocol for NEIL1 antibody 83376-7-RRDownload protocol
FC protocol for NEIL1 antibody 83376-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols