CoraLite® Plus 488-conjugated Nucleolin/C23 Polyclonal antibody
Nucleolin/C23 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-10556
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-10556 targets Nucleolin/C23 in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nucleolin/C23 fusion protein Ag0859 |
| Full Name | nucleolin |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC006494 |
| Gene Symbol | NCL |
| Gene ID (NCBI) | 4691 |
| RRID | AB_2918986 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Nucleolin/C23 antibody CL488-10556 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
