Nucleolin/C23 Polyclonal antibody

• Featured Product
• KD/KO Validated

Nucleolin/C23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

38

Reviews (1)

Cat no : 10556-1-AP

Synonyms

nucleolin, C23, Protein C23, NCL, FLJ45706

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Validation Data Gallery

• 

  WB analysis using 10556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10556-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10556-1-AP (Nucleolin/C23 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 10556-1-AP

  IP result of anti-Nucleolin/C23 (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (Nucleolin/C23 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 27 publications below
• IHC: See 4 publications below
• IF: See 15 publications below
• IP: See 4 publications below
• RIP: See 1 publications below

Product Information

10556-1-AP targets Nucleolin/C23 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nucleolin/C23 fusion protein Ag0859
• Full Name: nucleolin
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC006494
• Gene Symbol: NCL
• Gene ID (NCBI): 4691
• RRID: AB_2082423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Publications

Species	Application	Title
human	WB	Nature; C9orf72 nucleotide repeat structures initiate molecular cascades of disease.; Authors - Aaron R Haeusler
rat	IF	Neuron; Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.; Authors - Xinmei Wen
mouse	WB	Genome Res; The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.; Authors - Hui Fan
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen ; KD Validated
mouse	WB	Protein Cell; Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.; Authors - Chuan Chen
human,mouse	IF	EMBO J; The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.; Authors - Ella Doron-Mandel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

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