CoraLite® Plus 750-conjugated N4BP3 Recombinant antibody

N4BP3 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230547D12

Cat no : CL750-83178-2

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

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Validation Data Gallery

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  WB analysis using CL750-83178-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83178-2 (N4BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using CL750-83178-2

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 N4bp3 Recombinant Antibody (CL750-83178-2, Clone:230547D12)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83178-2 targets N4BP3 in WB, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: N4BP3 fusion protein Ag10194
• Full Name: Nedd4 binding protein 3
• Calculated Molecular Weight: 544 aa, 60 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC053323
• Gene Symbol: N4BP3
• Gene ID (NCBI): 23138
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.