N4BP3 Recombinant antibody

N4BP3 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230547D12

Cat no : 83178-2-RR

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

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Validation Data Gallery

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  WB analysis of MCF-7 using 83178-2-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83178-2-RR (N4BP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 83178-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 83178-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 83178-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug N4BP3 Recombinant antibody (83178-2-RR, Clone:230547D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83178-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83178-2-RR against Human N4BP3 were performed. The affinity constant is 66.3 pM.

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83178-2-RR targets N4BP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: N4BP3 fusion protein Ag10194
• Full Name: Nedd4 binding protein 3
• Calculated Molecular Weight: 544 aa, 60 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC053323
• Gene Symbol: N4BP3
• Gene ID (NCBI): 23138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.