1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 488 Mouse IgG2a isotype control (11A1B2) (CL488-66360-2, Clone:11A1B2)(red), or 0.25 ug CoraLite® Plus 488 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL488-65559, Clone: UCHT4) (green). Cells were not fixed, or unstained (blue). Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 488 Mouse IgG2a isotype control (11A1B2) (CL488-66360-2, Clone:11A1B2)(red), or 0.25 ug CoraLite® Plus 488 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL488-65559, Clone: UCHT4) (green). Cells were not fixed, or unstained (blue). Lymphocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL488-66360-2 targets IgG2a isotype control in FC applications and shows reactivity with megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 IgG2a isotype control antibody CL488-66360-2
1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 488 Mouse IgG2a isotype control (11A1B2) (CL488-66360-2, Clone:11A1B2)(red), or 0.25 ug CoraLite® Plus 488 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL488-65559, Clone: UCHT4) (green). Cells were not fixed, or unstained (blue). Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2a isotype control Monoclonal antibody
Catalog Number
CL488-66360-2
Citations
-
Dilutions
Applications
FC
Reactivity
megathura crenulata
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
