Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-Ig

Synonyms

IgG2a isotype, KLH, mouse IgG2a, mouse IgG2a control, Mouse IgG2a isotype control


"Mouse IgG2a isotype control Antibodies" Comparison

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Applications:
  • WB analysis using 66360-2-Ig

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 66360-2-Ig

    FC experiment of Jurkat using 66360-2-Ig

    1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2a isotype control antibody (66360-2-Ig, red) or control antibody (blue). Fixed with 90% MeOH.

  • FC experiment of HeLa using 66360-2-Ig

    FC experiment of HeLa using 66360-2-Ig

    1X10^6 HeLa cells were stained with 0.20ug Mouse IgG2a isotype control antibody (66360-2-Ig, red) or control antibody (blue).


Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66360-2-Ig targets Mouse IgG2a isotype control in WB, IP, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2a isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
FC protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Biosens Bioelectron

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Authors - Lei Wang
IP

Nature

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1

Authors - Milan Hluchý