1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG2a isotype control (AB-66360-2, Clone:11A1B2) (red), Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG2a isotype control (AB-66360-2, Clone:11A1B2) (red), Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
AB-66360-2 targets IgG2a isotype control in FC applications and shows reactivity with Megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
FC protocol for Atlantic Blue™ IgG2a isotype control antibody AB-66360-2
1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG2a isotype control (AB-66360-2, Clone:11A1B2) (red), Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
