Mouse IgG2a isotype control Monoclonal antibody, PBS Only

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-PBS

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

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Validation Data Gallery

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  WB analysis using 66360-2-Ig (same clone as 66360-2-PBS)

  Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66360-2-Ig (same clone as 66360-2-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-2-PBS targets Mouse IgG2a isotype control in WB, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG2a isotype control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).