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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Mitofilin Polyclonal antibody

Mitofilin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10179

Synonyms

DKFZp779P1653, HMP, IMMT, Mitochondrion Marker, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    IF Staining of HeLa using CL594-10179

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Mitofilin antibody (CL594-10179) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    FC experiment of HEK-293T using CL594-10179

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Mitofilin (CL594-10179) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10179 targets Mitofilin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mitofilin fusion protein Ag0102
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_2919722
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
IF protocol for CL594 Mitofilin antibody CL594-10179Download protocol
FC protocol for CL594 Mitofilin antibody CL594-10179Download protocol
Standard Protocols
Click here to view our Standard Protocols