IMMT Polyclonal antibody

• Featured Product
• KD/KO Validated

IMMT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

88

Reviews (6)

Cat no : 10179-1-AP

Synonyms

Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

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Validation Data Gallery

• 

  WB analysis using 10179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10179-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10179-1-AP

  IP result of anti-Mitofilin (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10179-1-AP (Mitofilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10179-1-AP (Mitofilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10179-1-AP (Mitofilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10179-1-AP (Mitofilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10179-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mitofilin antibody (10179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HEK-293T using 10179-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Mitofilin (10179-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, mouse brain tissue, MCF-7 cells, rat brain tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 77 publications below
• IHC: See 1 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

10179-1-AP targets IMMT in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMMT fusion protein Ag0102
• Full Name: inner membrane protein, mitochondrial (mitofilin)
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC002412
• Gene Symbol: IMMT
• Gene ID (NCBI): 10989
• RRID: AB_2127193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Publications

Species	Application	Title
human		Nat Methods; Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.; Authors - Mark Bates
human	WB	Cell Metab; Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism; Authors - Jee Hyung Sohn
mouse	WB	Cell Metab; A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.; Authors - Pedro Latorre-Muro
	WB	Cell Metab; The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.; Authors - Carmelina Petrungaro
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
human	WB	Mol Cell; The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation.; Authors - Katia Cosentino

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mia (Verified Customer) (09-28-2022)

very clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mitochondria protein

FH

siiting (Verified Customer) (03-20-2022)

very good antibody, second time buy already

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

FH

Maria (Verified Customer) (08-15-2021)

Mitofilin in human primary fibroblasts. Blocking with 5% milk in PBST (0.1%t tween) 1h RT. Primary ab 1:1000 in BSA 3% in PBST O/N at 4º incubation. Secondary 1:5000 HRP 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

IMMT Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:10179-1-AP)

FH

CYNTHIA (Verified Customer) (07-16-2020)

I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues

• Applications: Western Blot

FH

SITING (Verified Customer) (07-13-2020)

SHOW VERY STRONG SIGNAL

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Yan (Verified Customer) (01-28-2020)

This antibody worked very well on mouse heart tissue for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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