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Mitofilin Polyclonal antibody
Mitofilin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10179-1-AP
Validation Data Gallery
|Positive WB detected in||COLO 320 cells, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, mouse brain tissue, MCF-7 cells, rat brain tissue|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10179-1-AP targets Mitofilin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Mitofilin fusion protein Ag0102|
|Full Name||inner membrane protein, mitochondrial (mitofilin)|
|Calculated molecular weight||90 kDa|
|Observed molecular weight||80-90 kDa|
|GenBank accession number||BC002412|
|Gene ID (NCBI)||10989|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.
|Product Specific Protocols|
|WB protocol for Mitofilin antibody 10179-1-AP||Download protocol|
|IHC protocol for Mitofilin antibody 10179-1-AP||Download protocol|
|IF protocol for Mitofilin antibody 10179-1-AP||Download protocol|
|IP protocol for Mitofilin antibody 10179-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mia (Verified Customer) (09-28-2022)
very clear band
siiting (Verified Customer) (03-20-2022)
very good antibody, second time buy already
Maria (Verified Customer) (08-15-2021)
Mitofilin in human primary fibroblasts. Blocking with 5% milk in PBST (0.1%t tween) 1h RT. Primary ab 1:1000 in BSA 3% in PBST O/N at 4º incubation. Secondary 1:5000 HRP 1h RT.
CYNTHIA (Verified Customer) (07-16-2020)
I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues
SITING (Verified Customer) (07-13-2020)
SHOW VERY STRONG SIGNAL
Yan (Verified Customer) (01-28-2020)
This antibody worked very well on mouse heart tissue for western blot.