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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IMMT Polyclonal antibody

IMMT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10179

Synonyms

Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Mitofilin Polyclonal (CL488-10179)

    IF Staining of HepG2 using CL488-10179

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Mitofilin antibody (CL488-10179) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated Mitofilin Polyclonal (CL488-10179)

    FC experiment of HEK-293T using CL488-10179

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Mitofilin (CL488-10179) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10179 targets IMMT in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_2918958
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IMMT antibody CL488-10179Download protocol
FC protocol for CL Plus 488 IMMT antibody CL488-10179Download protocol
Standard Protocols
Click here to view our Standard Protocols