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CoraLite® Plus 488-conjugated MYCL1 Recombinant antibody

MYCL1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230104D6

Cat no : CL488-82881

Synonyms

Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated MYCL1 Recombinant an (CL488-82881)

    IF Staining of U2OS using CL488-82881

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 MYCL1 antibody (CL488-82881, Clone: 230104D6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MYCL1 Recombinant an (CL488-82881)

    FC experiment of HeLa using CL488-82881

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MYCL1 Recombinant antibody (CL488-82881, Clone:230104D6)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82881 targets MYCL1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYCL1 fusion protein Ag30335
Full Name v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
Calculated Molecular Weight 40 kDa
GenBank Accession NumberBC011864
Gene Symbol MYCL1
Gene ID (NCBI) 4610
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYCL1 antibody CL488-82881Download protocol
FC protocol for CL Plus 488 MYCL1 antibody CL488-82881Download protocol
Standard Protocols
Click here to view our Standard Protocols