MYCL1 Recombinant antibody

MYCL1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230104D6

Cat no : 82881-1-RR

Synonyms

Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38

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Validation Data Gallery

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  IF Staining of U2OS using 82881-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCL1 antibody (82881-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green).

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  FC experiment of HeLa using 82881-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYCL1 Recombinant antibody (82881-1-RR, Clone:230104D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82881-1-RR targets MYCL1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYCL1 fusion protein Ag30335
• Full Name: v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: BC011864
• Gene Symbol: MYCL1
• Gene ID (NCBI): 4610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.