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MUC20 Monoclonal antibody, PBS Only

MUC20 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, canine

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G9B2

Cat no : 68714-1-PBS

Synonyms

UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC20 Monoclonal antibody (68714-1-Ig)

    WB analysis using 68714-1-Ig (same clone as 68714-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68714-1-Ig (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68714-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MUC20 Monoclonal antibody (68714-1-Ig)

    IF Staining of HepG2 using 68714-1-Ig (same clone as 68714-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (68714-1-Ig, Clone: 1G9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68714-1-PBS in a different storage buffer formulation.

Product Information

68714-1-PBS targets MUC20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen MUC20 fusion protein Ag31840
Full Name mucin 20, cell surface associated
Calculated Molecular Weight 55 kDa, 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC029267
Gene Symbol MUC20
Gene ID (NCBI) 200958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).